Undergraduate Final Year Project
Thought of putting my work in the public domain.
Enhancement of laccase production in liquid cultures of Coriolopsis polyzona by different inducers.
Abstract
Coriolopsis polyzona is a white rot fungus that produces laccase enzyme, which belongs to the multicopper oxidase family. The enzyme has long been known for its ability to oxidise a wide range of substrates and has been employed in different industries. Although widely used, the production of the enzyme at industrial scale is limited by high production cost, low titres and long fermentation time and numerous approaches have been aimed at improving laccase levels. Various chemicals have been known to optimise laccase production at different concentrations. In the present study copper, 2,6–Dimethoxy phenol, 4–Aminobenzoic acid and isopropanol were investigated for their effect on laccase production in rapeseed cake liquid cultures of Coriolopsis polyzona. Laccase assay and protein assay were carried out to study laccase activity and total protein concentration in supplemented cultures. From all the chemicals, isopropanal was found to be the most potent inducer of laccase activity by giving 11-fold increase than the control. Also copper significantly enhances laccase activity by 125%, however both the aromatic compounds tested failed to effect laccase production.
